Hotspot

ndhC-trnV-UAC

LSC · 51616-52396 · 781 bp

Back to Angraecum

Pi 0.0222

MarkerSeek score 61.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 25
pi: 0.0222
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ATTGACTCCGCGAACAGTAC	TGAATCAGAGCACATGGAGC	50.0	50.0	58.0	58.0	1637-1780 bp	1.0	83.0
ndhC-trnV-UAC_p2	GGCTGTTAAGATGGGGTCTG	TGAATCAGAGCACATGGAGC	55.0	50.0	58.0	58.0	1490-1633 bp	1.0	82.9
ndhC-trnV-UAC_p3	CTTGGGCTCGATCTTGGTAC	TGAATCAGAGCACATGGAGC	55.0	50.0	58.1	58.0	1465-1608 bp	1.0	82.8
ndhC-trnV-UAC_p4	GCAAATAGGCATCCACAGGA	TGAATCAGAGCACATGGAGC	50.0	50.0	57.9	58.0	1665-1808 bp	1.0	82.5
ndhC-trnV-UAC_p5	ATTGACTCCGCGAACAGTAC	TTCAGATGCGAATTCCGGTT	50.0	45.0	58.0	57.9	1914 bp	0.333333	52.4