Hotspot

atpF-atpH

LSC · 14072-14246 · 175 bp

Back to Angraecum

Pi 0.0287

MarkerSeek score 69.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 175
variable_sites: 7
pi: 0.0287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAACTCGAAACTCCCAGCG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	295-297 bp	1.0	81.3
atpF-atpH_p2	TAAACTCGAAACTCCCAGCG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	57.9	58.3	284-286 bp	1.0	79.6
atpF-atpH_p3	CCACGGAAACAAAAGAATCGG	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	262-264 bp	1.0	73.6
atpF-atpH_p4	CCCACGGAAACAAAAGAATCG	ATTTATGGACTGGTCGTGGC	47.6	50.0	58.1	58.0	263-265 bp	1.0	73.5
atpF-atpH_p5	AAACTCGAAACTCCCAGCG	ATTTATGGACTGGTCGTGGC	52.6	50.0	58.1	58.0	294-296 bp	1.0	73.2