Hotspot

rps16-ACOS_CP_t003

LSC · 6204-7585 · 1382 bp

Back to Angophora

Pi 0
MarkerSeek score 58.80
Primer available yes
Label rps16-ACOS_CP_t003

Feature Metadata

label_name
rps16-ACOS_CP_t003
feature_type
igs
region
LSC
length_bp
1382
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-ACOS_CP_t003_p1 AGGGTGGGTGTAAGAATCCA GAAATTGAAATGGGGCGTGG 50.0 50.0 57.9 58.0 1550-1551 bp 1.0 79.6
rps16-ACOS_CP_t003_p2 AGGGTGGGTGTAAGAATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1492-1493 bp 1.0 79.0
rps16-ACOS_CP_t003_p3 GGGTGGGTGTAAGAATCCAC GAAATTGAAATGGGGCGTGG 55.0 50.0 57.9 58.0 1549-1550 bp 1.0 79.0
rps16-ACOS_CP_t003_p4 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTGG 45.0 50.0 58.1 58.0 1512-1513 bp 1.0 78.9
rps16-ACOS_CP_t003_p5 GGGTGGGTGTAAGAATCCAC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1491-1492 bp 1.0 78.5