Hotspot

matK-ACOS_CP_t002

LSC · 3709-4464 · 756 bp

Back to Angophora

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-ACOS_CP_t002

Feature Metadata

label_name
matK-ACOS_CP_t002
feature_type
igs
region
LSC
length_bp
756
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-ACOS_CP_t002_p1 ACTCTCGAAAAAGAAGTGGGT CGCTGTTTTGATTGCGTCAT 42.9 45.0 57.2 58.0 948 bp 1.0 65.0
matK-ACOS_CP_t002_p2 ACTCTCGAAAAAGAAGTGGGT AACGGTAGAGTACTCGGCTT 42.9 50.0 57.2 58.2 859 bp 1.0 63.9
matK-ACOS_CP_t002_p3 ACTCTCGAAAAAGAAGTGGGT ACGGTAGAGTACTCGGCTTT 42.9 50.0 57.2 58.2 858 bp 1.0 63.9
matK-ACOS_CP_t002_p4 ACTCTCGAAAAAGAAGTGGGT GGGTTGCTAACTCAACGGTA 42.9 50.0 57.2 57.5 872 bp 1.0 61.6
matK-ACOS_CP_t002_p5 ACTCTCGAAAAAGAAGTGGGT TTCGGGTTGCTAACTCAACG 42.9 50.0 57.2 58.5 875 bp 1.0 61.3