Hotspot

rps16

LSC · 12341-13407 · 1067 bp

Back to Angiopteris

Pi 0.00188

MarkerSeek score 54.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1067
variable_sites: 3
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGGGACAAGGAATACTCCAT	CTCTTGATGTGGTAAGAGTCGA	45.0	45.5	55.2	57.3	1262-1263 bp	1.0	40.3
rps16_p2	AGGGACAAGGAATACTCCAT	TCTCTTGATGTGGTAAGAGTCG	45.0	45.5	55.2	57.3	1263-1264 bp	1.0	40.3
rps16_p3	AGGGACAAGGAATACTCCAT	TGATGTGGTAAGAGTCGATGA	45.0	42.9	55.2	56.2	1258-1259 bp	1.0	40.3
rps16_p4	AGGGACAAGGAATACTCCAT	TCTTGATGTGGTAAGAGTCGA	45.0	42.9	55.2	56.1	1261-1262 bp	1.0	40.3
rps16_p5	AGGGACAAGGAATACTCCAT	CTTGATGTGGTAAGAGTCGATGA	45.0	43.5	55.2	58.0	1260-1261 bp	1.0	40.3