Hotspot

rpl16

LSC · 85067-86338 · 1272 bp

Back to Angiopteris

Pi 0.00105

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 2
pi: 0.00105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGAGAAAGAAATGTCTCAGGAACA	AGGATTAATAACAATTTGTCTCATTCT	37.5	25.9	58.1	55.7	1431-1432 bp	1.0	40.2
rpl16_p2	TGAGAAAGAAATGTCTCAGGAACA	AGGATTAATAACAATTTGTCTCAT	37.5	25.0	58.1	52.5	1431-1432 bp	1.0	40.2
rpl16_p3	TGAGAAAGAAATGTCTCAGGAACA	AGGATTAATAACAATTTGTCTCATTC	37.5	26.9	58.1	54.4	1431-1432 bp	1.0	40.2
rpl16_p4	TGAGAAAGAAATGTCTCAGGAACA	GGATTAATAACAATTTGTCTCATTCT	37.5	26.9	58.1	54.4	1430-1431 bp	1.0	40.2
rpl16_p5	TCGGTGAGAGAAAAATCTCTT	AGGATTAATAACAATTTGTCTCATTCT	38.1	25.9	54.6	55.7	1382-1383 bp	1.0	40.2