Hotspot

petA

LSC · 66824-67780 · 957 bp

Back to Angiopteris

Pi 0.00348

MarkerSeek score 48.50

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 957
variable_sites: 5
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TGTGATTGTTGAAACGGCAG	TCTCATTGCATTAACCATCACT	45.0	36.4	57.2	55.8	1130 bp	1.0	40.4
petA_p2	TGTGATTGTTGAAACGGCAG	ACGCTTTGACTAACTTAAGATTGG	45.0	37.5	57.2	57.5	1083 bp	1.0	40.4
petA_p3	GTGATTGTTGAAACGGCAGA	TCTCATTGCATTAACCATCACT	45.0	36.4	56.9	55.8	1129 bp	1.0	40.4
petA_p4	TTGTGATTGTTGAAACGGCAG	TCTCATTGCATTAACCATCACT	42.9	36.4	57.9	55.8	1131 bp	1.0	40.4
petA_p5	TGTGATTGTTGAAACGGCAG	TGTCGTACAAATTTTGAGAACCT	45.0	34.8	57.2	56.7	1161 bp	1.0	40.3