Hotspot

clpP

LSC · 73817-75641 · 1825 bp

Back to Angiopteris

Pi 0.000731

MarkerSeek score 58.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1825
variable_sites: 2
pi: 0.000731
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ACCTAACTCTGTACAAGATGGA	TCATTTTTCACAAGCTACGCT	40.9	38.1	55.9	56.5	1987-1988 bp	1.0	40.1
clpP_p2	ACCTAACTCTGTACAAGATGGAA	TCATTTTTCACAAGCTACGCT	39.1	38.1	56.7	56.5	1987-1988 bp	1.0	40.1
clpP_p3	ACCTAACTCTGTACAAGATGGA	TTCATTTTTCACAAGCTACGCT	40.9	36.4	55.9	57.2	1988-1989 bp	1.0	40.1
clpP_p4	ACCTAACTCTGTACAAGATGGA	TCATTTTTCACAAGCTACGC	40.9	40.0	55.9	54.9	1987-1988 bp	1.0	40.1
clpP_p5	ACCTAACTCTGTACAAGATGG	TCATTTTTCACAAGCTACGCT	42.9	38.1	54.4	56.5	1987-1988 bp	1.0	40.1