Hotspot

trnI-CAU-trnL-CAA

IRb · 89946-92463 · 2518 bp

Back to Angiopteris

Pi 0.00132
MarkerSeek score 57.50
Primer available yes
Label trnI-CAU-trnL-CAA

Feature Metadata

label_name
trnI-CAU-trnL-CAA
feature_type
igs
region
IRb
length_bp
2518
variable_sites
5
pi
0.00132
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnL-CAA_p1 TGAACCTGTGAATTCGCCAA GGTGAAATGGTAGACACGCA 45.0 50.0 58.0 58.2 2649-2922 bp 1.0 78.4
trnI-CAU-trnL-CAA_p2 TGAACCTGTGAATTCGCCAA TGGTGAAATGGTAGACACGC 45.0 50.0 58.0 58.2 2650-2923 bp 1.0 78.4
trnI-CAU-trnL-CAA_p3 TTGAACCTGTGAATTCGCCA GGTGAAATGGTAGACACGCA 45.0 50.0 58.0 58.2 2650-2923 bp 1.0 78.4
trnI-CAU-trnL-CAA_p4 TTGAACCTGTGAATTCGCCA TGGTGAAATGGTAGACACGC 45.0 50.0 58.0 58.2 2651-2924 bp 1.0 78.4
trnI-CAU-trnL-CAA_p5 TAACCGTTCAGCCATGGATG GGTGAAATGGTAGACACGCA 50.0 50.0 58.0 58.2 2612-2885 bp 1.0 78.3