Hotspot

petG-trnW-CCA

LSC · 70288-70428 · 141 bp

Back to Angiopteris

Pi 0.00473

MarkerSeek score 38.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 1
pi: 0.00473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GGTTGAAGCCCTGCTATCTG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.3	58.0	328 bp	1.0	77.5
petG-trnW-CCA_p2	GTTGAAGCCCTGCTATCTGG	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	58.3	58.0	327 bp	1.0	77.5
petG-trnW-CCA_p3	ATGGTTGAAGCCCTGCTATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.6	58.0	330 bp	1.0	77.3
petG-trnW-CCA_p4	GCCCTGCTATCTGGAATTGT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.6	58.0	321 bp	1.0	77.3
petG-trnW-CCA_p5	TGAAGCCCTGCTATCTGGAA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.4	58.0	325 bp	1.0	76.8