Hotspot

trnK-UUU-rps16

LSC · 11744-12340 · 597 bp

Back to Angiopteris

Pi 0.00223

MarkerSeek score 60.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 2
pi: 0.00223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAATCCCGTAGTCGCACTT	AAAGGAGCTCAAACTACCGC	45.0	50.0	57.5	58.2	732-733 bp	1.0	75.1
trnK-UUU-rps16_p2	AAAATCCCGTAGTCGCACTT	GAGCTCAAACTACCGCAACT	45.0	50.0	57.5	58.2	728-729 bp	1.0	75.0
trnK-UUU-rps16_p3	AGTCGCACTTAAAAGCCGAG	AAAGGAGCTCAAACTACCGC	50.0	50.0	58.6	58.2	722-723 bp	1.0	74.2
trnK-UUU-rps16_p4	AGTCGCACTTAAAAGCCGAG	GAGCTCAAACTACCGCAACT	50.0	50.0	58.6	58.2	718-719 bp	1.0	74.1
trnK-UUU-rps16_p5	TCGCACTTAAAAGCCGAGTA	AAAGGAGCTCAAACTACCGC	45.0	50.0	57.3	58.2	720-721 bp	1.0	73.0