Hotspot

trnH-GUG-psbA

LSC · 7459-7924 · 466 bp

Back to Angiopteris

Pi 0.0129

MarkerSeek score 55.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 9
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCTGTTGAAGCTCCTTCCG	50.0	50.0	57.9	57.8	525-526 bp	1.0	79.1
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	556-557 bp	1.0	79.0
trnH-GUG-psbA_p3	GATCCACAATCCACTGCCTT	GTAACGCTCACAACTTCCCT	50.0	50.0	57.9	57.8	569-570 bp	1.0	79.0
trnH-GUG-psbA_p4	GATCCACAATCCACTGCCTT	TTCTGTTGAAGCTCCTTCCG	50.0	50.0	57.9	57.8	538-539 bp	1.0	79.0
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	GTAACGCTCACAACTTCCCT	50.0	50.0	58.2	57.8	557-558 bp	1.0	78.8