Hotspot

petA-psbJ

LSC · 64311-65260 · 950 bp

Back to Angelica

Pi 0.0206

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 950
variable_sites: 90
pi: 0.0206
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1116-1187 bp	0.965517	79.3
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1103-1174 bp	0.965517	78.9
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1117-1188 bp	0.965517	78.6
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1104-1175 bp	0.965517	78.2
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	1121-1192 bp	0.965517	77.7