Hotspot

psaA-ycf3

LSC · 43431-44137 · 707 bp

Back to Angelica

Pi 0.00484

MarkerSeek score 67.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 30
pi: 0.00484
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	800-845 bp	1.0	85.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	787-832 bp	1.0	84.7
psaA-ycf3_p3	TTCTTGAGAAATGACCCGGC	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.2	58.1	823-868 bp	1.0	84.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	791-836 bp	1.0	84.5
psaA-ycf3_p5	TTCTTGAGAAATGACCCGGC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.2	57.8	810-855 bp	1.0	84.1