Hotspot

ccsA

Genome · 96769-97755 · 987 bp

Pi 0.0598

MarkerSeek score 77.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 987
variable_sites: 59
pi: 0.0598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGAAATGATAACTTTTTCGAATCA	ATTCACCGATCGGAATTGGG	25.0	50.0	53.6	58.0	1089 bp	0.5	20.0
ccsA_p2	TGAAATGATAACTTTTTCGAATCA	ATCGGAATTGGGAGGAGGTA	25.0	50.0	53.6	57.5	1081 bp	0.5	20.0
ccsA_p3	TGAAATGATAACTTTTTCGAATCA	TCGGAATTGGGAGGAGGTAT	25.0	50.0	53.6	57.5	1080 bp	0.5	20.0
ccsA_p4	TGAAATGATAACTTTTTCGAATCAATC	ATTCACCGATCGGAATTGGG	25.9	50.0	55.8	58.0	1089 bp	0.5	20.0
ccsA_p5	TGAAATGATAACTTTTTCGAATCA	GATCGGAATTGGGAGGAGGT	25.0	55.0	53.6	58.9	1082 bp	0.5	20.0