Hotspot

atpI

Genome · 17520-18302 · 783 bp

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Pi 0.0396
MarkerSeek score 66.60
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
Genome
length_bp
783
variable_sites
31
pi
0.0396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 ATTGCGATCCGAATGTGACA GCCGGGTCCTATAGAAGCTA 45.0 55.0 58.0 58.1 1567-1621 bp 1.0 87.0
atpI_p2 TGGCTGGGATTTTTGAACGA GCCGGGTCCTATAGAAGCTA 45.0 55.0 58.0 58.1 1771-1825 bp 1.0 86.5
atpI_p3 TGGCTGGGATTTTTGAACGA ACGGAAGCAGCAGAAATCAA 45.0 45.0 58.0 58.1 1725-1779 bp 1.0 86.5
atpI_p4 TGATGCCCGAGCCTCTATTA GCCGGGTCCTATAGAAGCTA 50.0 55.0 58.0 58.1 1578 bp 0.5 59.3
atpI_p5 TGATGCCCGAGCCTCTATTA ACGGAAGCAGCAGAAATCAA 50.0 45.0 58.0 58.1 1532 bp 0.5 59.1