Hotspot

rps15-ycf1

Genome · 104973-106728 · 1756 bp

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Pi 0.0794
MarkerSeek score 76.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
Genome
length_bp
1756
variable_sites
138
pi
0.0794
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AGATTCGACCGATCCATCCT CTGGCAAGGAAAGCGATTTG 50.0 50.0 58.0 58.0 2113-2144 bp 1.0 87.5
rps15-ycf1_p2 AGATTCGACCGATCCATCCT CTGCTACTTAACTGCCTGGG 50.0 55.0 58.0 58.0 2567-2589 bp 1.0 87.4
rps15-ycf1_p3 TCTCTGGGGCATTGACTGTA CTGGCAAGGAAAGCGATTTG 50.0 50.0 58.0 58.0 2617-2648 bp 1.0 86.0
rps15-ycf1_p4 ACGGATTGTGTGAAGCAAGT CTGGCAAGGAAAGCGATTTG 45.0 50.0 58.0 58.0 2082 bp 0.5 59.6
rps15-ycf1_p5 ACGGATTGTGTGAAGCAAGT CTGCTACTTAACTGCCTGGG 45.0 55.0 58.0 58.0 2536 bp 0.5 59.5