Hotspot

trnW-CCA-trnP-UGG

Genome · 66887-66935 · 49 bp

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Pi 0.146
MarkerSeek score 80.00
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
Genome
length_bp
49
variable_sites
7
pi
0.146
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGACATCAGGTTTTGGAGA GGTTCGAATCCTGTCATCCC 50.0 55.0 58.2 58.0 121-122 bp 1.0 83.4
trnW-CCA-trnP-UGG_p2 GCGACATCAGGTTTTGGAGA GGATGTAGCGCAGTTTGGTA 50.0 50.0 58.2 58.0 171-172 bp 1.0 82.1
trnW-CCA-trnP-UGG_p3 GTTTTGGAGACCTGCGTTCT GGTTCGAATCCTGTCATCCC 50.0 55.0 58.4 58.0 111-112 bp 1.0 82.1
trnW-CCA-trnP-UGG_p4 GCGACATCAGGTTTTGGAGA GCGCATTTGTTTTGGGTACA 50.0 45.0 58.2 57.9 151-152 bp 1.0 81.6
trnW-CCA-trnP-UGG_p5 GTTTTGGAGACCTGCGTTCT GGATGTAGCGCAGTTTGGTA 50.0 50.0 58.4 58.0 161-162 bp 1.0 80.6