Hotspot

psbE-petL

Genome · 65632-66315 · 684 bp

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Pi 0.105
MarkerSeek score 82.10
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
684
variable_sites
70
pi
0.105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATCAGCGAAAGGACGTTCTC ATCTAATTGGTCACCGCGTC 50.0 50.0 58.0 58.1 1112-1122 bp 1.0 88.3
psbE-petL_p2 ATCAGCGAAAGGACGTTCTC GATCTAATTGGTCACCGCGT 50.0 50.0 58.0 58.1 1113-1123 bp 1.0 88.3
psbE-petL_p3 ATCAGCGAAAGGACGTTCTC GGATGTAGCGCAGTTTGGTA 50.0 50.0 58.0 58.0 1412-1424 bp 1.0 88.0
psbE-petL_p4 ATCAGCGAAAGGACGTTCTC GGTTCGAATCCTGTCATCCC 50.0 55.0 58.0 58.0 1362-1374 bp 1.0 88.0
psbE-petL_p5 TCTAAGGAATTGAAGCGGCC GGATGTAGCGCAGTTTGGTA 50.0 50.0 58.0 58.0 1597-1609 bp 1.0 86.8