Hotspot

trnG-GCC-trnfM-CAU

Genome · 43550-43586 · 37 bp

Back to Aneura

Pi 0.162
MarkerSeek score 78.00
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
Genome
length_bp
37
variable_sites
6
pi
0.162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAATACGCGGGTTCGATTC TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.2 57.8 122-123 bp 1.0 81.6
trnG-GCC-trnfM-CAU_p2 AGAATACGCGGGTTCGATTC GTAGCTCGCAAGGCTCATAA 50.0 50.0 58.2 57.8 123-124 bp 1.0 81.5
trnG-GCC-trnfM-CAU_p3 GAGAATACGCGGGTTCGATT GTAGCTCGCAAGGCTCATAA 50.0 50.0 58.2 57.8 124-125 bp 1.0 81.5
trnG-GCC-trnfM-CAU_p4 GAGAATACGCGGGTTCGATT TAGCTCGCAAGGCTCATAAC 50.0 50.0 58.2 57.8 123-124 bp 1.0 81.5
trnG-GCC-trnfM-CAU_p5 CAAGGAGAATACGCGGGTTC TAGCTCGCAAGGCTCATAAC 55.0 50.0 58.7 57.8 127-128 bp 1.0 76.9