Hotspot

psbZ-trnG-GCC

Genome · 43231-43478 · 248 bp

Back to Aneura

Pi 0.164
MarkerSeek score 76.50
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
Genome
length_bp
248
variable_sites
40
pi
0.164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TGGTGGGTATTCTGAATTCATCT AATCGAACCCGCGTATTCTC 39.1 50.0 57.3 58.2 338-379 bp 1.0 58.0
psbZ-trnG-GCC_p2 TGGTGGGTATTCTGAATTCATCT GAATCGAACCCGCGTATTCT 39.1 50.0 57.3 58.2 339-380 bp 1.0 58.0
psbZ-trnG-GCC_p3 TTGGTGGGTATTCTGAATTCATCT AATCGAACCCGCGTATTCTC 37.5 50.0 57.9 58.2 339-380 bp 1.0 55.1
psbZ-trnG-GCC_p4 TTGGTGGGTATTCTGAATTCATCT GAATCGAACCCGCGTATTCT 37.5 50.0 57.9 58.2 340-381 bp 1.0 55.1
psbZ-trnG-GCC_p5 TGGTGGGTATTCTGAATTCA AATCGAACCCGCGTATTCTC 40.0 50.0 53.9 58.2 338-379 bp 1.0 55.0