Hotspot

trnT-GGU-psbD

Genome · 39524-40248 · 725 bp

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Pi 0.0816
MarkerSeek score 82.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
Genome
length_bp
725
variable_sites
59
pi
0.0816
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 CGGTTCGAATCTGGTAAGGG CTCAGCCAATCATCCATGCT 55.0 50.0 58.1 58.0 818-824 bp 1.0 85.3
trnT-GGU-psbD_p2 CGGTTCGAATCTGGTAAGGG AGACCAGACCAACCTACGAA 55.0 50.0 58.1 58.0 854-860 bp 1.0 85.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG CTCAGCCAATCATCCATGCT 55.0 50.0 58.1 58.0 853-859 bp 1.0 85.0
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG AGACCAGACCAACCTACGAA 55.0 50.0 58.1 58.0 889-895 bp 1.0 84.9
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA AGACCAGACCAACCTACGAA 50.0 50.0 58.1 58.0 892-898 bp 1.0 84.7