Hotspot

psbA-trnH-GUG

Genome · 30524-30707 · 184 bp

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Pi 0.16
MarkerSeek score 76.70
Primer available yes
Label psbA-trnH-GUG

Feature Metadata

label_name
psbA-trnH-GUG
feature_type
igs
region
Genome
length_bp
184
variable_sites
29
pi
0.16
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnH-GUG_p1 CCTCCGTCGAAGCTCCTG CTGCCTTAATCCACTTGGCT 66.7 50.0 59.5 57.9 245-247 bp 1.0 59.8
psbA-trnH-GUG_p2 CCTCCGTCGAAGCTCCTG GATCCACAATCCACTGCCTT 66.7 50.0 59.5 57.9 258-260 bp 1.0 59.8
psbA-trnH-GUG_p3 CCTCCGTCGAAGCTCCTG ACTGCCTTAATCCACTTGGC 66.7 50.0 59.5 58.2 246-248 bp 1.0 59.6
psbA-trnH-GUG_p4 CTCCGTCGAAGCTCCTGC CTGCCTTAATCCACTTGGCT 66.7 50.0 60.2 57.9 244-246 bp 1.0 54.3
psbA-trnH-GUG_p5 CTCCGTCGAAGCTCCTGC GATCCACAATCCACTGCCTT 66.7 50.0 60.2 57.9 257-259 bp 1.0 54.2