Hotspot

trnR-UCU-QU390_pgt004

Genome · 22120-22214 · 95 bp

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Pi 0.12
MarkerSeek score 80.70
Primer available yes
Label trnR-UCU-QU390_pgt004

Feature Metadata

label_name
trnR-UCU-QU390_pgt004
feature_type
igs
region
Genome
length_bp
95
variable_sites
11
pi
0.12
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-QU390_pgt004_p1 GGATTCGAACCTATACTCGAGG AGATGAGGCGTCGACTAGAA 50.0 50.0 57.6 58.0 225-234 bp 1.0 65.2
trnR-UCU-QU390_pgt004_p2 GGATTCGAACCTATACTCGAGG GATGAGGCGTCGACTAGAAC 50.0 55.0 57.6 57.9 224-233 bp 1.0 64.7
trnR-UCU-QU390_pgt004_p3 ACGTTCAATAGGATTCGAACCT AGATGAGGCGTCGACTAGAA 40.9 50.0 57.5 58.0 235-244 bp 1.0 64.6
trnR-UCU-QU390_pgt004_p4 GGATTCGAACCTATACTCGAGG CTAGAACCCCTAGCCTTCCA 50.0 55.0 57.6 57.8 211-220 bp 1.0 64.4
trnR-UCU-QU390_pgt004_p5 ACGTTCAATAGGATTCGAACCT GATGAGGCGTCGACTAGAAC 40.9 55.0 57.5 57.9 234-243 bp 1.0 64.1