Hotspot

ycf1

SSC · 125311-130764 · 5454 bp

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Pi 0.00342

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5454
variable_sites: 37
pi: 0.00342
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCACCCCTTTTGGTTCTTT	CAGAACTCGAACTTCCCAGA	45.0	50.0	57.4	56.9	337 bp	1.0	67.6
ycf1_p2	ACCACCCCTTTTGGTTCTTT	TCAGAACTCGAACTTCCCAG	45.0	50.0	57.4	56.9	338 bp	1.0	67.6
ycf1_p3	ACCACCCCTTTTGGTTCTTT	CAGAACTCGAACTTCCCAGAA	45.0	47.6	57.4	57.6	337 bp	1.0	65.4
ycf1_p4	ACCACCCCTTTTGGTTCTTT	GCACAGATACAAATTGAGGCC	45.0	47.6	57.4	57.6	258 bp	1.0	65.3
ycf1_p5	ACCACCCCTTTTGGTTCTTT	TCAGAACTCGAACTTCCCAGA	45.0	47.6	57.4	58.4	338 bp	1.0	65.2