Hotspot

rpl20-rps12

LSC · 69872-70633 · 762 bp

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Pi 0.00459

MarkerSeek score 62.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 7
pi: 0.00459
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATTTCCCCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	826-828 bp	1.0	74.8
rpl20-rps12_p2	TCCGAGCTATATTTCCCCGT	AGCGTCGAGGAACATGTACT	50.0	50.0	58.0	58.8	825-827 bp	1.0	74.0
rpl20-rps12_p3	TCCGAGCTATATTTCCCCGT	TCAGCGTCGAGGAACATGTA	50.0	50.0	58.0	58.8	827-829 bp	1.0	74.0
rpl20-rps12_p4	TCCGAGCTATATTTCCCCGT	GCGTCGAGGAACATGTACTA	50.0	50.0	58.0	57.2	824-826 bp	1.0	73.9
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	880-882 bp	1.0	73.9