Hotspot

petA-psbJ

LSC · 64202-65117 · 916 bp

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Pi 0.012

MarkerSeek score 55.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 22
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.1	58.1	1089-1106 bp	1.0	73.4
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.8	58.1	1076-1093 bp	1.0	73.0
petA-psbJ_p3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	50.0	47.6	58.1	57.8	1090-1107 bp	1.0	72.6
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	50.0	47.6	57.8	57.8	1077-1094 bp	1.0	72.3
petA-psbJ_p5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	55.0	47.6	57.7	58.1	1094-1111 bp	1.0	71.7