Hotspot

atpB-rbcL

LSC · 55826-56606 · 781 bp

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Pi 0.00257

MarkerSeek score 55.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 4
pi: 0.00257
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AGCCCCCGAACTAGAAGTAG	ACCCCAGCTTTGAATCCAAC	55.0	50.0	58.2	58.4	865-878 bp	1.0	76.3
atpB-rbcL_p2	AGCCCCCGAACTAGAAGTAG	CCCCAGCTTTGAATCCAACA	55.0	50.0	58.2	58.4	864-877 bp	1.0	76.3
atpB-rbcL_p3	AAGCCCCCGAACTAGAAGTA	ACCCCAGCTTTGAATCCAAC	50.0	50.0	57.8	58.4	866-879 bp	1.0	76.1
atpB-rbcL_p4	AAGCCCCCGAACTAGAAGTA	CCCCAGCTTTGAATCCAACA	50.0	50.0	57.8	58.4	865-878 bp	1.0	76.1
atpB-rbcL_p5	AGCCCCCGAACTAGAAGTAG	AACCCCAGCTTTGAATCCAA	55.0	45.0	58.2	57.6	866-879 bp	1.0	76.1