Hotspot

petN-psbM

LSC · 29516-30624 · 1109 bp

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Pi 0.00361

MarkerSeek score 48.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1109
variable_sites: 8
pi: 0.00361
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1283-1284 bp	1.0	68.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1222-1223 bp	1.0	67.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	58.2	57.2	1262-1263 bp	1.0	65.6
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1283-1284 bp	1.0	64.4
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	50.0	42.9	57.6	57.2	1201-1202 bp	1.0	64.1