Hotspot

trnC-GCA-petN

LSC · 28745-29425 · 681 bp

Back to Anethum

Pi 0.00441

MarkerSeek score 58.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 6
pi: 0.00441
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	55.0	50.0	57.9	58.2	744-777 bp	1.0	82.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	704-737 bp	1.0	82.8
trnC-GCA-petN_p3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	55.0	50.0	57.9	57.6	805-838 bp	1.0	81.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	765-798 bp	1.0	81.1
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	722-755 bp	1.0	80.6