Hotspot

rpoB-trnC-GCA

LSC · 27457-28673 · 1217 bp

Back to Anethum

Pi 0.00411

MarkerSeek score 42.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1217
variable_sites: 10
pi: 0.00411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTACATCTCGGAGC	CTTACCACTCGGCCATATCG	52.4	55.0	58.2	57.9	1262-1272 bp	1.0	70.3
rpoB-trnC-GCA_p2	CCCTCATTTACATCTCGGAGC	CCGGATTTGAACTGGGGAAA	52.4	50.0	58.2	58.1	1302-1312 bp	1.0	70.2
rpoB-trnC-GCA_p3	CCTCATTTACATCTCGGAGCA	CTTACCACTCGGCCATATCG	47.6	55.0	57.5	57.9	1261-1271 bp	1.0	68.6
rpoB-trnC-GCA_p4	CCTCATTTACATCTCGGAGCA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.5	58.1	1301-1311 bp	1.0	68.4
rpoB-trnC-GCA_p5	CCCTCATTTACATCTCGGAGC	TTATTGATCAGGCGACACCC	52.4	50.0	58.2	57.7	1320-1330 bp	1.0	68.2