Hotspot

atpH-atpI

LSC · 13946-15096 · 1151 bp

Back to Anethum

Pi 0.00796

MarkerSeek score 65.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1151
variable_sites: 18
pi: 0.00796
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	1213-1225 bp	1.0	81.0
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1205-1217 bp	1.0	80.8
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	1269-1281 bp	1.0	80.3
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1229-1241 bp	1.0	80.1
atpH-atpI_p5	AAGCGGCAGAAATCAATGGA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	1206-1218 bp	1.0	80.0