Hotspot

rps16-trnQ-UUG

LSC · 6369-7597 · 1229 bp

Back to Anethum

Pi 0.00448
MarkerSeek score 46.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1229
variable_sites
11
pi
0.00448
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1310-1312 bp 1.0 76.5
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.0 58.5 1311-1313 bp 1.0 76.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 1321-1323 bp 1.0 75.7
rps16-trnQ-UUG_p4 TGCTTTCTACCACAGCGTTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 1292-1294 bp 1.0 75.5
rps16-trnQ-UUG_p5 TTGCTTTCTACCACAGCGTT TCGGAGGTTCGAATCCTTTC 45.0 50.0 58.0 57.3 1293-1295 bp 1.0 75.5