Hotspot

rps19

LSC · 86093-86371 · 279 bp

Back to Anerincleistus

Pi 0.0133

MarkerSeek score 48.90

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 9
pi: 0.0133
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTGTCCCATAATCCCCCACT	CGTCGCCGTAGTAAATAGGG	50.0	55.0	58.0	58.0	931-935 bp	1.0	82.1
rps19_p2	TTGTCCCATAATCCCCCACT	GTTGGACAAGTGGGGAATGT	50.0	50.0	58.0	58.0	1171-1175 bp	1.0	82.0
rps19_p3	TTGTCCCATAATCCCCCACT	TGGACAAGTGGGGAATGTTG	50.0	50.0	58.0	58.0	1169-1173 bp	1.0	82.0
rps19_p4	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1032-1036 bp	1.0	81.8
rps19_p5	TTGTCCCATAATCCCCCACT	TAACATGAGTGATTCGGCGG	50.0	50.0	58.0	58.1	1532-1536 bp	1.0	81.0