Hotspot

rpoC2

LSC · 16825-20964 · 4140 bp

Back to Anerincleistus

Pi 0.00856

MarkerSeek score 47.50

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4140
variable_sites: 88
pi: 0.00856
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AGTTCCGTGATTTTGGTGGT	GGTTTGGTTCGAGCTTTCCT	45.0	50.0	57.6	58.4	192 bp	1.0	76.9
rpoC2_p2	AGTTCCGTGATTTTGGTGGT	TGGTTTGGTTCGAGCTTTCC	45.0	50.0	57.6	58.7	193 bp	1.0	74.6
rpoC2_p3	ATGTGCGAATAGTTCCGTGA	GGTTTGGTTCGAGCTTTCCT	45.0	50.0	57.3	58.4	202 bp	1.0	74.3
rpoC2_p4	TGTGCGAATAGTTCCGTGAT	GGTTTGGTTCGAGCTTTCCT	45.0	50.0	57.3	58.4	201 bp	1.0	74.3
rpoC2_p5	AGTTCCGTGATTTTGGTGGT	ACAAATGGTTTGGTTCGAGC	45.0	45.0	57.6	57.2	198 bp	1.0	73.6