Hotspot

rpoC1

LSC · 21132-23908 · 2777 bp

Back to Anerincleistus

Pi 0.00582

MarkerSeek score 50.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2777
variable_sites: 44
pi: 0.00582
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CACGCCAGTGTCACAAAATC	GCTTAATCGAAACGAATCGGA	50.0	42.9	58.0	56.8	2851-2867 bp	1.0	63.5
rpoC1_p2	CGCCAGTGTCACAAAATCAC	GCTTAATCGAAACGAATCGGA	50.0	42.9	58.0	56.8	2849-2865 bp	1.0	63.5
rpoC1_p3	CACGCCAGTGTCACAAAATC	AGCTTAATCGAAACGAATCGG	50.0	42.9	58.0	56.8	2852-2868 bp	1.0	63.4
rpoC1_p4	CGCCAGTGTCACAAAATCAC	AGCTTAATCGAAACGAATCGG	50.0	42.9	58.0	56.8	2850-2866 bp	1.0	63.4
rpoC1_p5	CGCCAGTGTCACAAAATCAC	AGCTTAATCGAAACGAATCGGA	50.0	40.9	58.0	58.2	2850-2866 bp	1.0	63.2