Hotspot

psbA

LSC · 330-1391 · 1062 bp

Back to Anerincleistus

Pi 0.00897

MarkerSeek score 51.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 24
pi: 0.00897
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AACACAAAACTACTGGCCGT	55.0	45.0	58.1	58.0	2050-2075 bp	0.857143	76.8
psbA_p2	GATCCACTTGGCTACATCCG	ACAAAACTACTGGCCGTGTT	55.0	45.0	58.1	58.0	2047-2072 bp	0.857143	76.8
psbA_p3	CAATCCACTGCCTTGATCCA	AACACAAAACTACTGGCCGT	50.0	45.0	57.9	58.0	2064-2089 bp	0.857143	76.7
psbA_p4	CAATCCACTGCCTTGATCCA	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2061-2086 bp	0.857143	76.7
psbA_p5	AATCCACTGCCTTGATCCAC	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2060-2085 bp	0.857143	76.7