Hotspot

atpE

LSC · 53939-54361 · 423 bp

Back to Anerincleistus

Pi 0.0128

MarkerSeek score 37.70

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 423
variable_sites: 12
pi: 0.0128
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	CGTTTATATAGAAGTTCCTTTGA	ACTGCGAAGGCTACGAATTT	30.4	45.0	52.1	57.9	490 bp	1.0	42.0
atpE_p2	CGTTTATATAGAAGTTCCTTTGA	ATGGTCTTCCTGAACAAGCC	30.4	50.0	52.1	57.8	540 bp	1.0	41.8
atpE_p3	CGTTTATATAGAAGTTCCTTTGA	AAATTGATCCTTTCCGGCGA	30.4	45.0	52.1	57.9	565 bp	1.0	41.7
atpE_p4	CGTTTATATAGAAGTTCCTTTGA	AATTGATCCTTTCCGGCGAA	30.4	45.0	52.1	57.9	564 bp	1.0	41.7
atpE_p5	CGTTTATATAGAAGTTCCTTTGA	TTGATCCTTTCCGGCGAATT	30.4	45.0	52.1	57.9	562 bp	1.0	41.7