Hotspot

petD-rpoA

LSC · 79764-79997 · 234 bp

Back to Anerincleistus

Pi 0.0301

MarkerSeek score 67.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 20
pi: 0.0301
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	317-318 bp	1.0	69.7
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	318-319 bp	1.0	69.7
petD-rpoA_p3	CATTTCGTCGTCCAGTAGCA	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.0	57.1	374-375 bp	1.0	69.5
petD-rpoA_p4	TTGATTGGTACTGTGGTGGC	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	342-343 bp	1.0	69.2
petD-rpoA_p5	CGTCCAGTAGCAACAACTGT	TCAGAAGCGATTTACCGAAGA	50.0	42.9	58.1	57.1	366-367 bp	1.0	68.6