Hotspot

petL-petG

LSC · 67319-67505 · 187 bp

Back to Anerincleistus

Pi 0.0331

MarkerSeek score 59.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 18
pi: 0.0331
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCATTTAGCCCCTTCATGCT	AGCGCTCTTAGTTCAGTTCG	45.0	50.0	56.9	58.0	617-624 bp	1.0	76.2
petL-petG_p2	TCATTTAGCCCCTTCATGCT	CAGAACGTGGGTCTCCAAAA	45.0	50.0	56.9	58.0	596-603 bp	1.0	76.2
petL-petG_p3	TCATTTAGCCCCTTCATGCT	AGAACGTGGGTCTCCAAAAC	45.0	50.0	56.9	58.0	595-602 bp	1.0	76.1
petL-petG_p4	TCATTTAGCCCCTTCATGCT	AAGAAAGGGATGTAGCGCAG	45.0	50.0	56.9	58.0	887-894 bp	1.0	75.2
petL-petG_p5	TCATTTAGCCCCTTCATGCT	GCTCTTAGTTCAGTTCGGCA	45.0	50.0	56.9	57.9	619-621 bp	0.714286	61.3