Hotspot

accD-psaI

LSC · 60183-61038 · 856 bp

Back to Anerincleistus

Pi 0.00912

MarkerSeek score 61.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 18
pi: 0.00912
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGTGTTCTGAGTGAGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	923-984 bp	1.0	71.7
accD-psaI_p2	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	922-983 bp	1.0	71.7
accD-psaI_p3	AAAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	42.9	45.0	57.1	58.0	923-984 bp	1.0	69.8
accD-psaI_p4	AAAGGGTGTTCTGAGTGAGT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	930-991 bp	1.0	67.3
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	AAAGACGCCATTGCAATTGC	45.0	45.0	56.3	58.6	929-990 bp	1.0	67.3