Hotspot

rbcL-accD

LSC · 58079-58706 · 628 bp

Back to Anerincleistus

Pi 0.0122

MarkerSeek score 70.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 21
pi: 0.0122
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	704-725 bp	1.0	80.9
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ATCCTCACTTCCACGAGTGT	50.0	50.0	57.9	58.4	793-814 bp	1.0	79.7
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACATCGAATTAAACCGCCGT	55.0	45.0	57.7	58.3	720-741 bp	1.0	79.4
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CACTTCCACGAGTGTCTTCA	50.0	50.0	57.9	57.5	788-809 bp	1.0	78.5
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ATCCTCACTTCCACGAGTGT	55.0	50.0	57.7	58.4	809-830 bp	1.0	78.2