Hotspot

trnS-GCU-trnR-UCU

LSC · 8011-9829 · 1819 bp

Back to Anerincleistus

Pi 0.0104

MarkerSeek score 66.10

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1819
variable_sites: 50
pi: 0.0104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1922-1963 bp	1.0	71.2
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1921-1962 bp	1.0	71.2
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1929-1970 bp	1.0	69.3
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1928-1969 bp	1.0	69.3
trnS-GCU-trnR-UCU_p5	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1923-1964 bp	1.0	69.2