Hotspot

rpl23

IRa · 157969-158256 · 288 bp

Back to Anemopaegma

Pi 0.0174
MarkerSeek score 56.60
Primer available yes
Label rpl23

Feature Metadata

label_name
rpl23
feature_type
gene
region
IRa
length_bp
288
variable_sites
16
pi
0.0174
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23_p1 ACTGTCTACGGTTCCATTGC ATGGAATGGAACGACGTAGG 50.0 50.0 57.9 57.4 482-585 bp 1.0 79.2
rpl23_p2 TGCTCGGGGTAGAAGTTTTG ATGGAATGGAACGACGTAGG 50.0 50.0 57.8 57.4 459-562 bp 1.0 78.8
rpl23_p3 GCTCGGGGTAGAAGTTTTGT ATGGAATGGAACGACGTAGG 50.0 50.0 57.8 57.4 458-561 bp 1.0 78.8
rpl23_p4 GTGCTCGGGGTAGAAGTTTT ATGGAATGGAACGACGTAGG 50.0 50.0 57.8 57.4 460-563 bp 1.0 78.8
rpl23_p5 ACTGTCTACGGTTCCATTGC AATGGAACGACGTAGGGATC 50.0 50.0 57.9 57.1 478-581 bp 1.0 77.0
rpl23_p1 ATGGAATGGAACGACGTAGG ACTGTCTACGGTTCCATTGC 50.0 50.0 57.4 57.9 482-585 bp 1.0 78.3
rpl23_p2 ATGGAATGGAACGACGTAGG TGCTCGGGGTAGAAGTTTTG 50.0 50.0 57.4 57.8 459-562 bp 1.0 77.8
rpl23_p3 ATGGAATGGAACGACGTAGG GCTCGGGGTAGAAGTTTTGT 50.0 50.0 57.4 57.8 458-561 bp 1.0 77.8
rpl23_p4 ATGGAATGGAACGACGTAGG GTGCTCGGGGTAGAAGTTTT 50.0 50.0 57.4 57.8 460-563 bp 1.0 77.8
rpl23_p5 AATGGAACGACGTAGGGATC ACTGTCTACGGTTCCATTGC 50.0 50.0 57.1 57.9 478-581 bp 1.0 76.0