Hotspot

clpP

LSC · 70216-72177 · 1962 bp

Back to Anemopaegma

Pi 0.0204

MarkerSeek score 55.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1962
variable_sites: 113
pi: 0.0204
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATAGCTGACCCTCGTAGTCC	ACGCAATGGGGGATTGATAC	55.0	50.0	58.0	58.0	2800-2990 bp	1.0	84.7
clpP_p2	GGATAGCTGACCCTCGTAGT	ACGCAATGGGGGATTGATAC	55.0	50.0	58.0	58.0	2802-2992 bp	1.0	84.7
clpP_p3	CTGGATAGCTGACCCTCGTA	ACGCAATGGGGGATTGATAC	55.0	50.0	58.0	58.0	2804-2994 bp	1.0	84.7
clpP_p4	TGGATAGCTGACCCTCGTAG	ACGCAATGGGGGATTGATAC	55.0	50.0	58.0	58.0	2803-2993 bp	1.0	84.7
clpP_p5	TCTTTGGCTCTACCCCTGAA	ACGCAATGGGGGATTGATAC	50.0	50.0	58.0	58.0	2893-2959 bp	0.75	73.8