Hotspot

ndhF-rpl32

SSC · 117993-118433 · 441 bp

Back to Anemopaegma

Pi 0.0154
MarkerSeek score 56.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
441
variable_sites
20
pi
0.0154
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCACGCATATTGATATGTCTGT TGATGCAGAAGTACGTTTTT 39.1 35.0 57.8 53.5 500-502 bp 1.0 43.8
ndhF-rpl32_p2 TCCACGCATATTGATATGTCTGT TTGATGCAGAAGTACGTTTT 39.1 35.0 57.8 53.5 501-503 bp 1.0 43.8
ndhF-rpl32_p3 TCCACGCATATTGATATGTCTGT TTTGATGCAGAAGTACGTTT 39.1 35.0 57.8 53.5 502-504 bp 1.0 43.8
ndhF-rpl32_p4 TCCACGCATATTGATATGTCTGT TTTTGATGCAGAAGTACGTT 39.1 35.0 57.8 53.5 503-505 bp 1.0 43.8
ndhF-rpl32_p5 TCCACGCATATTGATATGTCTGT TTTTTGATGCAGAAGTACGT 39.1 35.0 57.8 53.5 504-506 bp 1.0 43.8