Hotspot

trnN-GUU-ycf1

IRb · 107962-108828 · 867 bp

Back to Anemopaegma

Pi 0.0428

MarkerSeek score 73.20

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 867
variable_sites: 79
pi: 0.0428
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AAATCGTGATCGGGATAGCG	ACGACAACGACCGAATTGAT	50.0	45.0	57.9	57.9	919-1467 bp	0.875	81.7
trnN-GUU-ycf1_p2	AAATCGTGATCGGGATAGCG	GCCCTATGGAAAATGCGGTA	50.0	50.0	57.9	58.0	1033-1508 bp	0.5	64.5
trnN-GUU-ycf1_p3	GTGTGGGTCTTCAGCGTATT	GCCCTATGGAAAATGCGGTA	50.0	50.0	57.9	58.0	991-1466 bp	0.5	64.2
trnN-GUU-ycf1_p4	TTAACGGTCAAAGTGAGCCC	GCCCTATGGAAAATGCGGTA	50.0	50.0	58.1	58.0	646-1121 bp	0.5	64.1
trnN-GUU-ycf1_p5	GGTAAACGCGCTTTCACATC	GCCCTATGGAAAATGCGGTA	50.0	50.0	58.1	58.0	1253-1722 bp	0.5	64.1