Hotspot

psaA-ycf3

LSC · 41627-42612 · 986 bp

Back to Anemopaegma

Pi 0.0155

MarkerSeek score 68.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 986
variable_sites: 42
pi: 0.0155
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.1	58.0	1057-1165 bp	1.0	85.9
psaA-ycf3_p2	CCCATGCCTCGAAAGAAGTT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.2	58.0	1037-1145 bp	1.0	85.3
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	AGGCTATAGCGCTTACTCCT	50.0	50.0	58.1	57.7	1051-1159 bp	1.0	83.9
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	TGGTTGAAGATCACGAGACG	50.0	50.0	58.1	57.7	1007-1115 bp	1.0	83.6
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	TTGGAAACAGGCTATAGCGC	50.0	50.0	58.1	58.3	1059-1167 bp	1.0	83.5