Hotspot

trnK-UUU-rps16

LSC · 4270-5176 · 907 bp

Back to Anemopaegma

Pi 0.0124
MarkerSeek score 60.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
907
variable_sites
32
pi
0.0124
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 1220-1244 bp 1.0 83.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 1219-1243 bp 1.0 83.0
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1623-1647 bp 1.0 82.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1622-1646 bp 1.0 82.6
trnK-UUU-rps16_p5 GGGAGAAGTCCTTCCCGTAT AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.2 58.0 1983-2007 bp 1.0 81.9