Hotspot

ycf1

SSC · 125802-131438 · 5637 bp

Back to Anemone

Pi 0.0218

MarkerSeek score 60.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 436
pi: 0.0218
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCCCGATTAGTTTGGTGC	AGGCCCAAAGGATTCCCTAT	50.0	50.0	57.9	58.1	1077-1113 bp	0.909091	84.1
ycf1_p2	ATCCCCGATTAGTTTGGTGC	GGGGCCAGTGCTAACATTAA	50.0	50.0	57.9	57.9	871-901 bp	0.909091	83.7
ycf1_p3	TCCCATTTTCATATCGCGCA	GGGGCCAGTGCTAACATTAA	45.0	50.0	58.0	57.9	937-967 bp	0.818182	79.5
ycf1_p4	TCCCATTTTCATATCGCGCA	AGGCCCAAAGGATTCCCTAT	45.0	50.0	58.0	58.1	1143-1179 bp	0.818182	79.3
ycf1_p5	TCCCATTTTCATATCGCGCA	AATGAGGCCCAAAGGATTCC	45.0	50.0	58.0	57.8	1147-1183 bp	0.818182	78.5